ABSTRACT
Objective To study the effect of a new immunomodulator composed of muramyl dipeptide(MDP) and anti-CD10monoclonal antibody(MDP-Ab)on the dendritic cells(DC)of children with acute leukemia. Methods DC was adopted to divide the children with acute lymphoblastic leukemia into 6 groups,including the control group,unconjugated anti-CD10alone,unconjugated MDP alone,MDP-Ab alone,lipopolysaccharide(LPS)alone and MDP-Ab + LPS.The immunophenotypes,the endocytosis interleukin-12(IL-12)were detected.The stimulation index of autologous lymphocytes was assayed by adopting 5-(and 6)-carboxyfluorescein diacetate,succinimidyl es-ter(CFSE)-staining method.The supernatants of DC and autologous lymphocytes were used to detect the level of in-terferon-γ(IFN-γ)by using enzyme-linked immunosorbent assay.Results (1)DC immunophenotype:The ex-pressions of human leukocyte antigen-DR(HLA-DR),mature molecule(CD83)and co-stimulatory molecules (CD80and CD86)were increased significantly upon DC triggered with MDP-Ab,compared with the control group,un-conjugated anti-CD10group,and unconjugated MDP group,but lower than those in LPS and combination of MDP-Ab with LPS(F=629.62,P=0.000).(2)The level of IL-12:a significant increase in IL-12 level was detected in MDP-Ab group,LPS group,and combination of MDP-Ab with LPS group,compared with the control group,uncon-jugated anti-CD10group,and unconjugated MDP group(F=857.87,P=0.000). There were significant differences among the first three groups.(3)Endocytosis assay:The uptake of DCs stimulated by unconjugated anti-CD10,un-conjugated MDP,MDP-Ab immunoconjugate,LPS or combination was lower than that of immature DC in the control group which was(81.3 ± 10.1)%.(4)Mixed lymphocyte reaction and IFN-γ level:DC,treated with MDP-Ab, LPS and combination,stimulated more CFSE positive cells and higher level of IFN-γ secretion than the control group and unconjugated anti-CD10group,unconjugated MDP group. The most significance was observed in combination of MDP-Ab with LPS(F=393.36,P=0.000;F=2 497.18,P=0.000).Conclusion It is concluded that MDP-Ab could promote the proliferation and maturation of DC derived from blood of children with acute leukemia.
ABSTRACT
Objective To explore the effects of hydrocolloid dressing on neonatal scleredema.Methods Forty patients with neonatal scleredema were enrolled from Department of Neonatology,Affiliated Hospital of Qingdao University from February 2015 to February 2017.The patients were assigned to the experimental group and the control group by random number table with 20 cases in each group.The experimental group was treated with hydrocolloid dressing besides routine comprehensive treatment,and the hydrocolloid dressing was changed every 3～4 d until swelling disappeared.The control group was treated with routine comprehensive treatment.Body temperature,response and swelling of two groups were observed.Results For therapeutic effects between two groups,the total effective rate of the experiment group was 95％,and was significantly better than that of the control group which was 65％(P＜0.05).The recovery time of body temperature and the time of swelling disappear in the experiment group were 38.25±17.33 h and 3.05±1.00 d;while those in the control group were 57.35±32.21 h and 4.65±1.57 d,and the differences were statistically significant(P＜0.05).Conclusion Applying hydrocolloid dressing at swelling site in premature infants can significantly shorten the time of skin swelling,promote body temperature recovery,and improve treatment effects.
ABSTRACT
Objective To explore the efficacy of fluconazole mouthwash by means of oxygen-driven atomizing inhalation for treating the pediatric oral fungal infections. Methods A total of 60 cases of child patient from the department of Pediatrics of Jiaozhou People's Hospital have been selected and divided into control group and test group with 30 cases for each group by the method of random number table. The sodium bicarbonate solutions as well as the fluconazole capsules were used for the two groups of children patients. Everyday there were five doses of sodium bicarbonate solution as follows:one after getting up in the morning, one after breakfast/lunch/dinner, and one before bedtime by gargling for three minutes followed by spitting. Daily dose of fluconazole capsules was 3 mg/kg, with 50 ml of NS added for preparing a solution of 1-3 mg/ml. Differences between the two groups are as follows. The control group used the traditional method of gargling. The prepared fluconazole solution was divided into five doses for gargling followed by swallowing which were carried out two hours after the sodium bicarbonate solution. The test group used the oxygen-driven atomizing inhalation therapy. The intervals with the sodium bicarbonate solution were two hours. Before the oxygen-driven atomizing inhalation therapy, the children patients had gargled with water to remove the food debris inside the mouth. Next, the daily dose was divided into five doses to be added into the nebulizers for carrying out the oxygen-driven atomizing inhalation therapy focusing on the exposed ulcer areas. The difference in healing time of oral fungal infection of different position and degree between both groups of child patient has been observed. Results The healing time of oral mucositis at different position of both groups has been compared with each other, with the exception of gingival position, the healing time of child patients in test group at the position of cheek, palate, tongue, pharynx, lips and so on was respectively (6.67±1.68) d, (6.38±1.80) d, (6.36±1.96) d, (6.50±1.60) d and (5.00±0.82) d, which were all better than that of the control group, which was (8.13 ± 2.13) d,(8.00 ± 2.14) d,(8.23 ± 2.13) d, (8.67 ± 1.75) d and (7.20 ± 1.48) d, the difference between both groups was statistically significant(t=2.14-2.64, P<0.05);the healing time of child patients in test group with a degree ranging from I to IV was respectively (5.10±0.88) d, (7.92±1.32) d,(8.00±1.00 )d and (10.25±0.96)d,which were all better than that of the control group, which was(6.36±0.81)d,(9.00±1.29) d,(10.33 ± 0.58)d and(12.33 ± 0.58)d,the difference between both groups was statistically significant(t=2.10-3.50,P<0.05). Conclusions It's suitable for oral care of children patients if we replace the fluconazole mouthwash with the oxygen-driven atomizing inhalation therapy which can reduce the severity of oral mucositis and shorten the healing time.